General Cell Based Assays
Eurofins Discoverx PathHunter Human GPR85 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR85 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR85 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Genscript Corporation DPPIV/CD26 hFc Chimera Human
CD26/dipeptidyl peptidase (DPP)IV is a membrane-bound protein found in many cell types of the body and a soluble form is present in body fluids There is longstanding evidence that various primary tumors and also metastases express DPPIV/CD26 to a variable extent
Eurofins Discoverx PathHunter Mouse SDF-1 alpha (CXCR4) β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Mouse SDF-1 alpha (CXCR4) β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure CXCR4 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress IL22RA1/IL10RB Dimerization Assay, 2pl Kit. (93-1026E1CP7M)
The PathHunter IL22RA1/IL10RB Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-22) induced dimerization of IL22RA1/IL10RB receptors. This product uses EFC technology. The cell line included overexpresses IL22RA1 target protein fused to PK alongside IL10RB target protein fused to EA. Upon ligand-induced dimerization of IL22RA1/IL10RB, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
AURORA BIOLABS LLC Kras Q61H Nucleotide Exchange Assay Kit,100 rxn
KRAS is a small GTPase in the RAS protein family that plays a critical role in regulating cell signaling pathways involved in proliferation, differentiation, and survival. KRAS cycles between an inactive GDP-bound state and an active GTP-bound state, and activation occurs through nucleotide exchange from GDP to GTP. Mutations such as KRAS Q61H are frequently associated with human cancers and contribute to abnormal signaling and uncontrolled cell growth. The KRAS Q61H Nucleotide Exchange Assay is a TR-FRET–based assay designed to quantitatively monitor nucleotide exchange activity of KRAS Q61H. The assay detects changes in fluorescence resonance energy transfer generated during nucleotide exchange, enabling measurement of KRAS activation and evaluation of compounds that modulate KRAS signaling for cancer research and drug discovery applications.
Meso Scale Discovery R-PLEX Human IL-22R 2 ASY
The Meso Scale Discovery R-PLEX Human IL-22Ralpha2 Assay is a multiplex immunoassay that measures IL-22Ralpha2 in human serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements The assay can be multiplexed with other R-PLEX assays to analyze multiple biomarkers simultaneously This allows efficient and accurate quantification of IL-22Ralpha2 levels alongside other immune response markers With a testing time of just 3 5 hours this assay enables rapid profiling of IL-22Ralpha2
Eurofins Discoverx PathHunter Human RANK/IkB Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human RANK IkB Stable Cell Line Functional Kinase Assay (U2OS) measures pathway activation-induced degradation of IκB target protein. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery U-PLEX Mouse IL-9 ASY
The Meso Scale Discovery U-PLEX Mouse IL-9 Assay Kit is an electrochemiluminescent immunoassay for the quantitative measurement of mouse interleukin 9 (IL-9) in serum plasma and cell culture supernatants The assay uses MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and reproducible quantitation of mouse IL-9 with a lower limit of detection of 0 3 pg/mL and an upper limit of 10 000 pg/mL The kit includes a 96-well plate coated with capture antibody detection antibody conjugated to MSD SULFO-TAG calibrators and sample diluent The assay takes approximately 3 5 hours to complete after a 2 hour incubation
Revvity Health Sciences Inc AlphaScreen FITC Detection Kit, 50,000 Assay Points
AlphaScreen FITC Detection Kit, 50,000 Assay Points
STEMCELL Technologies Ascorbic Acid, Size: 100 g
Support the proliferation of mesenchymal stem cells (Choi et al. J Biosci Bioeng, 2008) with Ascorbic Acid. Ascorbic Acid is a reducing agent that shows antioxidant activity against reactive oxygen species. Ascorbic Acid is used to differentiate mouse embryonic stem (ES) cells into osteoblasts (zur Nieden et al. Differentiation, 2003) and cardiomyocytes (Takahashi et al. Circulation, 2003). This small molecule also promotes differentiation of osteoblasts from human and mouse mesenchymal cells (Pittenger et al. Science, 1999; Tropel et al. Exp Cell Res, 2004) and increases the efficiency of reprogramming mouse and human fibroblasts to induced pluripotent stem (iPS) cells (Esteban et al. Cell Stem Cell, 2010). L-Ascorbic Acid is supplied as a crystalline solid with ≥95% purity; Molecular weight 176.1 g/mol; CAS Number 50-81-7. Visit STEMCELL.com for more information and related products.
Eurofins Discoverx PathHunter Human EphA4 Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human EphA4 Stable Cell Line Functional Kinase Assay (U2OS) measures EphA4 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery VPLEX GLP1 Total Kit 25 PL
The Meso Scale Discovery V-PLEX GLP-1 Total Kit is an electrochemiluminescent immunoassay for the quantitative measurement of total glucagon-like peptide-1 (GLP-1) in human serum plasma and cell culture supernatants The assay utilizes MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and reproducible results with a broad dynamic range and rapid protocol This kit includes a 96-well plate coated with capture antibody detection antibody conjugated to ruthenium label calibrators and reagents required to perform the assay The lower limit of detection is 1 5 pg/mL with an upper limit of 10 000 pg/mL
Eurofins Discoverx PathHunter MC4R Bioassay Kit, 10-Plate. (93-0211Y3-00086)
The PathHunter MC4R Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. αMSH) based activation of MC4R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MC4R and EA-tagged β-Arrestin-2. Activation of MC4R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Abcam Aldolase Activity Assay Kit (Colorimetric)
Aldolase Activity Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter Human NF-kB Pathway Reporter Cell-Based Assay Kit (U2OS)
The PathHunter Human NF-kB Pathway Reporter Stable Cell Line Assay (U2OS) of induced expression levels of a tagged reporter protein. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.